Prof. Brian Dale

SCIENTIFIC PRODUCTIVITY 1973-2009
Peer Reviewed Scientific papers –107
Books – 7
Patents – 4
Cecchi MD, Cohen J, Dale B: Filtering system and method for incubators.
United States Patent 6,013,119. Jan 11, 2000.
Cecchi MD, Cohen J, Dale B: Volatile organic compound and particulate filter assembly for
purifying a gas stream.
United States Patent 6, 200, 362 B1, March 16, 2001.
Cecchi MD, Cohen J, Dale B: Connection device for connecting a filtering system to an incubator.
United States Patent 6, 225, 110, B1, May 1, 2001.
Wilding M., and Dale, B ( pending USA)
PUBLICATIONS
Dale, B. (1973)- Blood pressure and its hydraulic functions in Helix pomatia L .J. exp. Biol., 59 :
477-490.
Dale, B. (1974a) – The eco-physiological significance of the circulatory mechanics of Lymnaea
stagnalis L. Comp. Biochem. Physiol. 47A : 1105-1113.
Dale,B. (1974b) – Extrusion, retraction and respiratory movements in Helix pomatia in relation to
distribution and circulation of the blood. J.Zool.,Lond. 173 : 427-439.
Dale, B. Denis-Donini, S. DeSantis, R., Monroy A and Taglietti, V. (1978) Sperm-egg interactions
in the ascidians. Biol. Cellulaire 32: 129-133.
Dale,B., De Felice, L.J. and Taglietti, V. (1978) – Membrane Noise and conductance increase
during single spermatozoon-egg interactions. Nature. Lond. 275 : 217-219.
Dale,B. (1979) – Radation response of an intraspecific somatic cell hybrid (L5178Y,X-ray resistant
line X L5178Y, X-ray sensitive line). Radiat. Res. 79 : 338-348.
De Felice, L.J. and Dale, B. (1979) – Voltage response to Fertilization and Polyspermy in Sea
Urchin Eggs and Oocytes. Develop. Biol. 72 : 327-341.
Dale, B., De Santis A., and Hoshi, M. (1979) – Membrane response to 1-methyl-adenine requires
the presence of the nucleus. Nature Lond. 282 : 89-90.
Dale, B. and Monroy, A. (1981) – How is polyspermy prevented; a critical review. Gamete Res. 4:
151-169.
Dale, B. and De Santis A. (1981a) – The effect of cytochalasin b and d on the fertilization of sea
urchins. Develop.Biol. 83: 232-237.
Dale, B. and De Santis A: (1981b) – Maturation and fertilization of the sea urchin oocyte: an
electrophysiological study. Develop. Biol. 85: 474-484.
Dale, B., Dan-Sohkawa, M., De Santis, A. and Hoshi, M. (1981) – Fertilization of the starfish
Astropecten aurantiacus. Exp. Cell. Res. 132 : 505-510.
Dale, B., De Santis A. and Hagstrom, B.E. (1982) – The effect of nicotine on sperm-egg interaction
in the sea urchin; polyspermy and electrical events. Gamete Res. 5: 125 – 135.
Dale, B., De Santis A., Ortolani G., Rasotto M. and Santella L. (1982) – Electrical coupling of
blastomeres in early embryos of ascidians and sea urchins. Exp. Cell Res. 140: 457-461.
Dale, B., De Santis A. and Ortolani G., (1983) – Electrical response to fertilization in ascidian
oocytes. Develop.Biol. 99, 188-193.
Dale, B. and De Felice, L.J. (1984) – Sperm activated channels in ascidian oocytes. Develop. Biol.
101 : 235-239.
Monroy, A., Rosati, F. and Dale, B. (1984) – Sperm-egg interaction. Boll. Zool. 51; 103-119.
Dale, B.(1994) – Book Review. “Mechanism and Control of Animal Fertilization”, Ed. J.F.
Hartmann. Quart.Rev.Biol. 59; 177-178
Dale, B. and Russo, P. (1984) – Electrical response to fertilization in starfish eggs and oocytes.
Cell.Biol.Int.Rep.8 : 1015.
Dale, B., De Felice, L.J. and Ehrenstein, G. (1985) – Injection of a soluble sperm fraction into sea
urchin eggs triggers the cortical reaction. Experientia 41 : 1068-1070.
Dale, B. and Santella, L. (1985) – Sperm – oocyte interaction in the sea urchin. J. Cell Sci. 74 : 153-
167.
Dale, B. (1985) – Sperm receptivity in sea urchin oocytes and eggs. J.Exp.Biol. 118: 85-97.
Talevi, R., Dale, B., and Campanella, C. (1985) – Fertilization and activation potentials in
Discoglossus pictus (Anura) eggs: A delayed response to activation by pricking. Develop.Biol.111:
316-323.
Talevi, R. and Dale, B. (1986) – Electrical characteristics of Ascidian egg fragments. Exp. Cell.
Res. 162 : 539-543.
De Felice, L., Dale, B. and Talevi, R. (1986) – Distribution of fertilization channels in ascidian
oocyte membranes. Proc.R.Soc. Lond. B. 229: 209-214.
Ciccarelli, C., Russo, P., De Santis, A. and Dale B. (1987) – pH in the jelly layer of starfish
oocytes. Develop. Growth Differ. 29: 257-261
Dale, B. (1987) – Mechanism of Fertilization. Nature Lond. 325; 762-763
Dale, B. (1987) – Fertilization channels in ascidian eggs are not activated by Ca. Exp. Cell. Res.
172: 474-480.
De Santis, A., Ciccarelli, C. and Dale, B. (1987) – Free cations in echinoderm oocytes and eggs.
Eur. Biophysics Journal 14: 471-476.
Dale,B. and Russo, P. (1988) – Sulfhydryl groups are involved in the activation of sea urchin eggs.
Gamete Res. 19 : 161-168.
Dale, B. (1988) – Primary and secondary messengers in the activation of ascidian eggs. Exp. Cell
Res. 177 : 205-211.
Dale, B. and Talevi, R. (1989) – Distribution of ion channels in ascidian eggs and zygotes. Exp.
Cell. Res. 181 : 238-244.
Dale, B., Hagstrom, B. and Santella, L. (1989) – Partially fertilized sea urchin eggs: An
electrophysiological and morphological study. Develop. Growth Differ. 31: 165-170.
Russo, P., Pecorella, M., de Santis, A. and Dale, B. (1989) pH in eggs of the ascidian Ciona
Intestinalis at fertilization and activation. J. Exp. Zool. 250: 329-332.
Marthy, J. and Dale, B. (1989) – Dye-coupling in the early squid embryo. Rouxs Archivs. Dev Biol.
198: 211-218.
Dale, B. (1989) – Fertilization in Ascidians. In “ Non-mammalian models for biomedical research “
Ed. A. Woodhead. CRC Press, Boca Raton.
Dale , B. (1990) –Fertilization in acquatic animals. In “ Experimental Embryology in aquatic plants
and animals “. Ed H.J. Marthy, Nato ASI Series A: Life Sciences Vol. 195, Plenum, New York.
Dale, B. and De Felice L. (1990) – Souble sperm factors, electrical events and egg activation. In “
Mechanism of Fertilization: Plants to Humans “. Ed B. Dale, Nato ASI Cell Biology Series H. Vol
45. Springer Verlag, Heidelberg.
Brownlee, C. and Dale, B. (1990) – Temporal and spatial correlation of fertilization current,
calcium waves and cytoplasmic contraction in eggs of Ciona Intestinalis. Proc. R. Soc. Lond. 239 :
321-328.
Dale, B. (1991) – Mechanism of Fertilization. In “ Cell to Cell Signals in Plants and Animals “ Ed
V. Neuhoff and J. Friend, NATO ASI Series H, Cell Biology Vol. 51, Springer Verlag, Heidelberg.
Dale, B., De Felice, L. and Talevi, R. (1991). – L-type calcium currents in ascidian eggs. Exp. Cell
Res. 192:302-306
Dale, B., Santella, L., and Tosti, E. (1991).- Gap junctional permability in early and cleavage
arrested ascidian embryos. Development 112: 153-160.
Dale, B., Gualtieri, R., Talevi, R., Tosti, E., Santella, L. and Elder, K. (1991) – Intercellular
communication in the early human embryo. Mol. Reprod. Develop. 29: 22-28.
Tosti, A and Dale, B. (1992) Lithium and phorbol ester modify the activating capacity of ascidian
spermatozoa. Experientia, 48, 57-60.
Gualtieri, R., Santella, L. and Dale B. (1992). Tight junctions and cavitation in the human preembryo.
Molec. Rprod. Develop. 32:81-87.
Santella, L., Alikani, M., Talansky, B.E., Cohen, J., and Dale, B.(1992). Is the human oocyte
plasma membrane polarized? Human Rprod. 7: 999-1003.
Tosti, E., Palumbo, A., and Dale, B. (1993). Inositol tri-phosphate in human and ascidian
spermatozoa. Molec. Reprod. Develop. 35 :52-56.
Pecorella, C. Tosti, E., Kyozuka, K and Dale, B. (1993) Activation currents sperm entry and surface
contractions in ascidian oocytes. Zygote.1.113-119.
Dale, B., De Felice,L., Kyozuka, K., Santella, L. and Tosti, E. (1994). Voltage Clamp of the nuclear
envelope. Proc R. Soc. Lond. B. 255, 119-124.
Tosti, E. and Dale, B. (1994). Regulation of the Fertilization Current in Ascidian Oocytes by
intracellular Second Messengers. Molec. Rprod. Develop. 37, 473-476.
Yazaki, I., Tosti, E. and Dale, B (1995). Cytoskeletal elements link calcium channel activity and the
cell cycle in early sea urchin embryos. Development. 121;1827-1831.
Dale, B., Iaccarino, M., Fortunato, A., Gragnaniello, G., Kyozuka, k. And Tosti, E. (1994). A
morphological and functional study of fusibility in round headed spermatozoa in the human. Fert.
Ster. 61, 336-340.
Gianaroli, L. Tosti, E., Magli, C., Iaccarino, M., Ferraretti, A. and Dale, B. (1994).
The Fertilization Current in the Human Oocyte. Molec. Reprod. Develop. 38, 209-214.
Dale, B. (1994) – Oocyte activation in invertebrates and human. Zygote. 2, 373-377.
Fiorentino, A., Magli, M., Fortini, D., Feliciani, e:, Ferraretti, A., Dale, B. and Gianaroli, L.(1994).
Sperm-oocyte ratios in an invitro fertilization (IVF) Program . J. Assist. Reprod. Genetics. 11,97-
103.
Dale, B., Tosti, E., and Iaccarino, M(1995). Is the plasma membrane of the human oocyte reorganized
following fertilization and early cleavage. Zygote, 3, 31-37.
Menezo, Y. and Dale, B. (1995). Paternal contribution to successful embryogenesis. Human
Reproduction. 10, 1326-1328.
Wiemer, K., Dale, B., Hu, Y., Steuerwald, N., Maxson, W. And Hoffman, D. (1995). Blastocyst
Development in co-culture: Development and Morphological aspects. Human Reproduction, 10,
3226-3232.
Dale, B. (1996) – Fertilization. In “ Human Physiology: From Cellular Mechanisms to Intregation
“. Eds.R.Greger, H.Koepchen, W. Mommaerts. Spinger Verlag, Heidelberg.
Dale, B (1996) “Reproduction, Artificial Insemination Processes“ in. Enciclopedia Italiana
Treccani, Rome, Italy
Russo, G., Kyozuka, K., Antonazzo, L., Tosti, E. and Dale, B.(1996). Maturation promoting Factor
in ascidian oocytes is regulated by different intracellular signals at meiosis 1 and 11. (Development,
122, 1995-2003).
Dale, B., Monfrecola, V., Fortunato, A. and Tosti, E.(1996). A soluble sperm factor gates calcium
activated K+ channels in human oocytes. J. Assist Reprod. Genetics, 13, 573-577.
Gianaroli, L., Magli,C., Ferraretti, A., Fiorentino, A., Panzella, S., Tosti, E., and Dale, B. (1996).
Reducing the time of sperm-oocyte interaction in human IVF improves the implantation rate.
(Human Reproduction, 11, 166-171).
Dailey, T., Dale, B., Cohen, J. and Munne’, S. (1996). Association between Nondisjunction and
Maternal Age in Meiosis II Human Oocytes. Am. J. Hum. Genet 59, 176-184.
Wilding, M. and Dale, B., (1997). Sperm Factor, what is it and what does it do? Mol. Hum.
Reprod., 3, 269-273.
Dale,B., Yazaki,I and Tosti, E (1997) Polarized distribution of L-type calcium channels in early sea
urchin blastomeres Am. J. Physiol. 273, 822-825.
Wilding, M., Kyozuka, K., G.L., Tosti, E. and Dale, B., (1997). A souble extract from human
spermatozoa activates ascidian eggs. Dev, Growth Diff., 39, 329-336
Grumetto, L., Wilding, M., De Simone, M. L.., Tosti, E., Galione, A. and Dale, B. (1997). Nitric
oxide triggers the fertilisation current in ascidian. Biochem, Byophisy. Res. Comm. 239, 723-728.
Cohen, Gilligan, A Esposito, W., Schimmel, T and , Dale B. (1997) Ambient air and its otential
effects on conception in vitro. Human Reproduction. 12, 1742-1746.
Menezo Y., Dale, B. and Guerin, P. (1997) Co-culture and embryo development: Biochemical
aspects. In “ In vitro fertilization and assisted reproduction. Proc. Xth World Congress on in vitro
fertilization and assisted reproduction. Vancouver pp147-154.
Russo, G.L., Wilding, M., Marino, M. and Dale, B. (1998). Ins and outs of meiosis in ascidians.
Sem. Cell Dev. Biol. 9, 559-567.
Menezo Y and Dale, B (1998) Mammalian embryo quality: is it possible to estimate it and when?
J.Assisted Reprod. Gen 15, 458-460.
Menezo Y. Hamamah, S Hazout, A and Dale, B. (1998) Time to switch from co-culture to
sequential defined media for transfer at the blastocyst stage. Human reprod. 13, 2043-2044.
Wilding, M., Menezo, Y., Cohen, J. And Dale, B. (1998). Regulation of intracellular pH in
mammalian preimplantation embryos. Ass. Reprod.Rev
Wilding, M., Russo, G.L., Marino M., Grumetto L. DeSimone M., Tosti, E and Dale, B. (1998)
Acvtivation of Ciona intestinalis at fertilization is controlled by nicotinamide nucleotide
metabolism. In “ New developments in Marine Biotechnology” (Eds LeGal and Halvorson) Plenum
Press, New York. Pp121-123.
Russo, G.L., Kyozuka, K., Marino M., Tosti, E , Wilding, M. DeSimone, M and Dale, B. (1998)
Meiotic cell cycle control by mos in ascidian oocytes. In “ New developments in Marine
Biotechnology” (Eds LeGal and Halvorson) Plenum Press, New York. Pp121-123.
De Simone, M.L., Grumetto, L., Tosti, E., Wilding, M. and Dale, B. (1998). Non-specific currents
at fertilization in sea urchin eggs. Zygote 6,11-15
Wilding, M., and Dale, B. (1998). Soluble extracts from ascidian spermatozoa trigger intracellular
calcium release independently of the activation of the ADP ribose channel. Zygote 6, 149-154.
Wilding, M., Russo, G.L., Marino, M., Dale, B., and Galione, A. (1998). ADP ribose gates the
fertilization current in ascidian oocytes. Am. J. Physiol. 275, C1277-C1283
Dale, B.., Menezo, Y., Cohen, J., Di Matteo, L. and Wilding, M. (1998). Intracellular pH regulation
in the human oocyte. Hum. Reprod. 13, 964-970.
Dale, B., Marino, M. and Wilding, M. (1999). Sperm-induced calcium oscillations. Mol. Hum.
Reprod. 5,1-4.
Dale,B., Fiorentino, A., DeStefano, R., DiMatteo, L., Wilding, M. and Zullo, F. (1999). Pregnancies
after activated oocyte transfer: a new alternative for infertility treatment. Human Reproduction. 14,
1771-1772.
Dale, B., Di Matteo, L., Marino, M., Russo., G. and Wilding, M.(1999). Soluble sperm activating
factors. In “ The Male Gamete: From Basic Knowledge to Clinical Applications”. Cache River
Press. Il, USA
Boni, R. Tosti, E., Roviello, S and Dale, B (1999) Intercellular communication in in vivo and in
vitro produced bovine embryos. Biology of Reproduction. 61, 1050-1055.
Yazaki,I, Dale, B and Tosti,E. (1999). Functional gap junctions in the Early Sea Urchin Embryo are
localised to the vegetal pole. Developmental Biology. 212, 508-510.
Dale, B., Marino, M. and Wilding M (1999). The Ins and Outs of Meiosis. J. Exp. Zool. 285, 226-
236.
Marino, M., Wilding, M. and Dale, B. (2000). Interaction of cell cycle kinases, microtubules and
chromatin in ascidian oocytes during meiosis. Molec.Reprod. Develop. 56, 155-162.
Wilding, M.,Marino, M.,Monfrecola, V. Dale, B.(2000). Meiosis associated calcium waves in
ascidian oocytes are correlated with the position of the male centrosome. Zygote, 8, 285-293.
Wilding, M. Dale, B. Marino, M. DiMatteo, L. Alviggi, G. DePlacido, G. (2001). Mitochondrial
Aggregation patterns and activity in human oocytes and pre-implantation embryos. Human
Reproduction,16, 909-917.
Dale, B. Wilding M., Botta, G, Marino, M., DiMatteo, L., DePlacido, G., Izzo, A. (2001) Pregnancy
after cytoplasmic transfer to reduce embryo fragmentation in a couple suffering from idiopathic
infertility. Human Reproduction ,16, 1469-1472.
Wilding, M., Carotenuto, R., Infante, V, Dale, B., Marino, M., DiMatteo, L., Campanella, C.
(2001). Confocal microscopy analysis of the activity of mitochondria contained within the
mitochondrial cloud during oogenesis in Xenopus laevis. Zygote 9, 347-352.
Cigliano, L., Spagnuolo, M., Dale, B., Balestrieri, M., Abrescia P. (2001). Estradiol esterification in
the human pre-ovulatory follicle. Steroids, 66,889-896.
Balestrieri, M.,Cigliano, L. DeSimone, M., Dale, B., Abrescia, P. (2001) Haptoglobin, inhibits
Lecithin.-Cholesterol Acyltransferase activity in human ovarian follicular fluid. Molecular
Reproduction and Development , 59, 186-191.
Cigliano, L., Balestrieri, M., Spagnuolo, M., Dale, B. Abrescia, P. (2002). Lecithin Cholesterol
Acyltransferase Activity during maturation of Human Pre-ovulatory Follicles with different
concentrations of ascorbate, a-tocopherol and nitrotyrosine. Reprod.Fertil.Dev.14,, 15-21.
De Placido, G. Wilding, M., Strina I., Mollo, A., Alviggi, E Tolino A, Colacurci N., De Matteo L.
Marino M and Dale, B. (2002). The effect of ease of transfer and type of catheter used on
pregnancy and implantation rates in an IVF program. J.Assisted Reproduction and Genetics. 19, 14-
18.
Wilding., M. Fiorentino A., DeSimone ML., Infante V. DeMatteo, L., Marino M and Dale, B.
(2002). Energy substrates, the mitochondrial membrane potential and human pre-implantation
embryo division. Repro. Biomed.Online. 5, 39-42.
Dale, B., Wilding., M.DiMatteo L. and Zullo F. (2002). Case Report. Absence of sperm sex
chromosome aneuploidies in an XO/XYY male. Fertility and Sterility, 78, 634-636.
Dale, B., Fiorentino, A., DeSimone, M., DiMatteo, L., Marino, M., Wilding, M., Fehr, P., Bassan
E., Maselli, M., Cappiello, F., Zullo, F. (2002).Zygote versus embryo transfer: a propective
randomised multicentre trial. (J Ass. Reprod. and Genetics ., 19, 456-461.
De Placido, G., Wilding, M., Strina, I, Alviggi, E., Mollo, A, Vricchio, M., Tolino, A., Schiattarella,
C, Dale B (2002). High outcome predictability After IVF using a combined score for zygote and
embryo morphology and growth rate., Human Reproduction17, 2402-2409.
Wilding, M. DePlacido, G., DiMatteo, L., Marino,M. Alviggi, C. and Dale, B. (2003)Chaotic
mosaicism in human pre-implantationembryos is correlated with a low membrane mitochondrial
membrane potential. Fertility and Sterility., 79: 340-346.
Wilding, M., Forman, R. Hogewind G., DiMatteo, L. Zullo F., Cappiello., and Dale, B. (2004). Preimplantation
genetic diagnosis for the treatment of failed IVF-ET and habitual abortion. Fertility
and Sterility. 81,1302-1307.
Wilding, M., Di Matteo, L. and Dale, B(2005). The maternal age effect in human assisted
reproduction: a hypothesis based on oocyte metabolism. Zygote 13, 317-323.
Scotto di Frega, Dale, B., DiMatteo, L and Wilding, M. (2005). Secondary male factor infertility
after Roux-en-Y gastric bypass for morbid obesity: case report. Human Reproduction. 20, 997-998.
Wilding M., Singer, M. Fehr, P., Haberlin, F., Roth, F. Lachat, R., DiMatteo,L., .Capobianco C.,
Dale B. (2006) The effect of extended culture of cumulus-oocyte complexes in follicular fluid
during in vitro fertilization cycles. J.Assist Reprod.Genet. 23(3) 129-36.
Wilding, M., DiMatteo, L., D’Andretti, S., Montanaro, N., Capobianco, C., Dale B. (2007) An
oocyte score for use in assisted reproduction . J.Assist Reprod Genet 24, 350-358.
Dale, B., DiMatteo, L., Wilding, M. (2007). Mitochondria in Reproduction: Future assays for
embryo selection. in “ Human Preimplantation Embryo Selection” Eds Elder Kay and Cohen
Jacques. pp 275-285. Informa Healthcare.
BOOKS
Dale, B. (1983) – “Fertilization in animals”. The Institute of Biology’s studies in Biology No. 157
(Ed. Edward Arnold, London).
Dale, B. (1990) – “ Mechanism of Fertilization: Plants to Humans “. Nato ASI Cell Biology Series
H, Vol 45. Springer Verlag, Heidelberg.
Tosti, E. and Dale, B (1995) – “Fecondazione in vitro “ Edizioni Scientifiche Italiano, Milan, Italy.
Dale, B. and Elder, K. (1997) – “ In vitro Fertilization” Cambridge University Press. Cambridge,
UK.
Dale, B. (1998) Development of the Human Embryo in Vitro. Human Reproduction Vol 13,
Supplement 4. Oxford University Press.UK
Elder, K. and Dale, B (2000) – “ In vitro Fertilization 2nd Ed”. Cambridge University Press.
Cambridge, UK.
Dale, B. (in press). Fertilization and Early Development: From Gametes to Embryos. Cambridge
University Press.